anti-Drebrin mouse monoclonal, MX823, supernatant
- Hybridoma cell culture supernatant
- Mouse monoclonal
- Suitable for ICC/IF, IHC frozen and WB
- Reacts with bovine, dog, human, mouse, rat
- Isotype: IgG1
|Application||ICC/IF, IHC, IP, WB|
|Purification||Hybridoma cell culture supernatant|
|Reactivity||Bovine, Dog, Human, Mouse, Rat|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Synthetic c-terminal peptide (aa632-649) coupled to KLH|
|Formulation||Contains 0.09% sodium azide|
|UniprotID||Q16643 8 (Human)|
|Synonym||Developmentally-regulated brain protein, DBN1
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (IHC) - frozen||1:10-1:50|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:100|
MX823 specifically reacts with drebrin, a widespread actin-associating protein of 70 kD (SDS-PAGE mobility shows Mr 120,000). It is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells. Positive staining was found in many tissues, including diverse epithelia and carcinoma (e.g. in epidermis basal cells are positive, whereas overlying cells are negative; also positive: regenerating epithelium during wound healing; basal cell carcinoma; skin melanoma), specific types of endothelia and smooth muscle; especially prominent in the mesangial cells of renal glomeruli and in the Sertoli cells of testis.
Also positive: epithelia of hair follicles and eccrine sweat glands. Consistently negative were, however, hepatocytes and cross-striated muscle.
Reactivity on cultured cell lines: primary human keratinocytes, U333, MCF-7, SV80, B16 (mouse melanoma cells).
Q & A's
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The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.