Disease related

anti-p97 ATPase mouse monoclonal, 58.13.3, supernatant concentrate

Lyophilized

Supernatant

Cat. No. 65278

Available, delivery time 1-7 days

€212.00*

Key Features
  • Hybridoma cell culture supernatant concentrate
  • Mouse monoclonal
  • Suitable for WB
  • Reacts with human, rabbit, rat and xenopus
  • Isotype: IgG1
Product description
Quantity 500 µl
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application WB
Purification Hybridoma cell culture supernatant concentrate
Reactivity Human, Rabbit, Rat, Xenopus
No reactivity Budding yeast Cdc48p
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone 58.13.3
Immunogen 15S Mg2+ ATPase complex purified from Xenopus laevis ovary
Formulation Contains 0.09% sodium azide
Synonym VCP, valosin-containing protein
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Not recommended
Immunoprecipitation (IP) Not recommended
Western Blot (WB) 1:2,000-1:5,000
Background

p97 ATPase/VCP (also described as valosin-containing protein) is implicated in homotypic membrane fusion events and is related to the N-ethyl maleimide-sensitive fusion protein NSF/Sec18p.
The antibody reacts with the p97 subunit of 15S Mg2+-ATPase. p97/VCP is involved in ubiquitin-proteasome dependent protein degradation processes and involvement was also found e.g. in the formation of neuronal inclusion bodies in neuro-degenerative diseseases (e.g. Parkinson's disease).

Positive control: immunoblots with protein extracts from all vertebrate tissues and cells.

Reactivity on cultured cell lines: all vertebrate cells and tissues tested so far: e.g. Xenopus laevis; rabbit, rat, human; does not crossreact with the corresponding protein in budding yeast (Cdc48p).

References/Publications (5)
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Category Protocol
Size 919.99 KB
Filetype pdf
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