AAV9 VP1 + VP2 + VP3, recombinant proteins, set

Cat. No. 72009

Available, delivery time 1-7 days

€753.00*

Key Features
•    Recombinant AAV9 VP1-3 capsid protein set
•    Useful for ratio analysis
•    Precise molar concentration
•    Suitable for WB, dot blot, SDS PAGE

Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.


Included products:

Product description
Quantity 10 µg each protein
Application Dot blot, SDS PAGE, WB
Purification Ni-NTA chromatography
Storage -80°C
Intended use Research use only
Concentration 100 µg/ml (VP1: 1.19 µM, VP2: 1.45 µM, VP3: 1.61 µM)
Formulation Liquid, 6 M urea in PBS
Source Escherichia coli
Molecular weight VP1: ~90 kDa, VP2: ~68 kDa, VP3: ~62 kDa
Purity > 95% (determined by SDS PAGE)
Product description N-terminal His-tagged recombinant AAV9 capsid proteins VP1 + VP2 + VP3
Applications
Tested applications Tested dilutions
Dot Blot 100 ng, depending on primary antibody and detection method
SDS PAGE 1 µg
Western Blot (WB) 5-20 ng, depending on primary antibody and detection method
Background

The AAV capsid consists of three capsid proteins, i.e. VP1, VP2 and VP3, which differ in their N-terminus and encapsulate the genomic ssDNA. In native virus particles, the three proteins form subunits with a ratio of 1:1:10 (VP1:VP2:VP3), in a total number of 60 subunits per capsid.
This set of recombinant AAV9 VP1, VP2 and VP3 can be used to create a mixture with the precise molar ratio of 1:1:10 to compare the protein composition of the viral capsid in your sample by protein detection methods, e.g. western blot.
All three recombinant AAV9 capsid proteins are available as set (Cat. No. 72009) or as individual proteins (Cat. No. 640842, 640843, 640844).

Note: please find an example how to prepare western blot samples in the pipetting scheme below. Aliquots of the remaining samples can be stored at -80°C for reuse.

Set content:
Cat. No. 640842 AAV9 VP1, recombinant protein
Cat. No. 640843 AAV9 VP2, recombinant protein
Cat. No. 640844 AAV9 VP3, recombinant protein
Downloads
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.


Related Products

AAV9 ELISA Control
Cat. No. PRAAV9-C
  • Consists of fully assembled, empty AAV9 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

€110.00*
AAV9 empty capsids
Cat. No. 66V090
  • Fully assembled, empty AAV9 capsids
  • Serotype-specific conformational epitopes
  • Reliable positive control for dot blot, western blot, ELISA
  • Quality controlled for purity, AAV titer and filling grade
  • Alignment to internal reference standard material
Not available in China and in the Russian Federation.
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.

€269.00*
AAV9 Titration ELISA
Cat. No. PRAAV9
  • ELISA for quantitation of AAV9 capsids
  • Detection of full and empty AAV9 capsids
  • ADK9 antibody is used as capture and detection antibody

€560.00*
AAV9 Xpress ELISA
Cat. No. PRAAV9XP
Fast, easy and reliable AAV9 titer determination:
  • get results in less than 2 hours and increase your daily sample throughput
  • easy workflow & process integration
  • same accuracy and reproducibility as the standard AAV9 Titration ELISA
  • ADK9 antibody is used as capture and detection antibody

€685.00*
anti-AAV VP1 mouse monoclonal, A1, lyophilized, purified
Cat. No. 61056
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with VP1 of AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9 and AAVDJ
  • Isotype: IgG2a

€382.00*
anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, liquid, purified
Cat. No. 690058
Quantity: 1 ml
  • Mouse monoclonal
  • Suitable for affinity chromatography, dot blot, ICC/IF, IP and WB
  • Reacts with VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
  • Isotype: IgG1

Variants from €110.00*
€382.00*
anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum
Cat. No. 61084
  • Rabbit polyclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAVDJ and AAVrh74, weakly with AAV4
  • Strong affinity for denatured capsid proteins
  • Weak affinity for native capsids

€382.00*
anti-AAV9 (intact particle) mouse monoclonal, ADK9, liquid, purified
Cat. No. 690162
Quantity: 1 ml (50 µg/ml)
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for dot blot, ELISA, ICC and neutralization assay
  • Reacts with AAV9 intact particles
  • Isotype: IgA kappa

Variants from €110.00*
€447.00*
anti-AAV9 (intact particle) mouse recombinant, ADK9-1R, lyophilized, purified
Cat. No. 610178
Quantity: 50 µg
  • Purified, lyophilized
  • Recombinant antibody
  • Suitable for dot blot, ELISA and neutralization assay
  • Reacts with AAV9 intacts particles
  • Isotype: mouse IgG1

Variants from €110.00*
€447.00*
Dip’n’Check AAV9 - lateral flow assay
Cat. No. PR5209
  • Rapid lateral flow test for the detection of AAV9 capsids
  • Semi-quantitative AAV titer determination in cell lysates and purified preparations
  • Detection of full and empty AAV9 capsids

€163.00*
OptiPrep™ - Iodixanol
Cat. No. 1893 (previous 1114542)
  • Highly effective for the separation of full and empty AAV particles
  • Designed for the purification of viruses (such as AAV)
  • Useful to isolate different cell types and organelles
  • Ready-made, sterile and endotoxin-tested solution of iodixanol

€178.00*