Recognize fully assembled
capsids easily

 

AAV particle antibodies recognize conformational epitopes on viral capsids that are only present on fully assembled capsids. These epitopes are made up of distant amino acids either from the same protein or from different proteins that are close by.

You can use particle antibodies to

Analyze: track the location of virus particles and identify protein-protein interactions
Purify: suitable for AAV particle purification using affinity chromatography
Characterize: determine your AAV capsid titers
Control: analyze the condition of your AAV particles
Monitor: use as a positive control for neutralization assays with patient sera

 

View All AAV Particle Antibodies

Cross Reactivity & Neutralization

 

All our AAV particle antibodies were tested for their cross-reactivity and some showed high cross-reactivity with other serotypes. Use the table below to see the cross reactivity.

Click on the buttons in the first column to get more product information including specific binding sites, neutralizing activity and available products.

Antibody Clone
AAV1
AAV2
AAV3
AAV4
AAV5
AAV6
AAV7
AAV8
AAV9
AAVrh10
AAV11
AAAV12
AAVrh74
Anc80
AAVDJ
AAV2.7m8
ADK1a
                  na na   na
   A20   
                    na na  
  A20R  
                       
  ADK4  
                      na na   na
   ADK5a
                      na na   na
  ADK5b 
          na       na na na na   na
  ADK6   
                      na na   na
   ADK8   
            (✓)       na
 ADK8/9 
            na (✓) na na (✓) na
   ADK9                          na na na na
ADK9-1R
            na     na na na na na na


✓ - Reactivity
(✓) - Weak Reactivity
na - Not analysed
Empty field - no reactivity

Data for assembled capsids AAV7/11/12 Under Courtesy of Regine Heilbronn, Charité Universitätsmedizin Berlin
Mietzsch et al. Hum Gene Ther. 2014 Mar 1; 25(3):212 222

What customers are saying


'All AAV primary antibodies I have ordered from PROGEN have been specific and reliable.'

Scientist I, ElevateBio

AAV Antibodies FAQs

Yes, our AAV particle antibodies detect conformational epitopes only present on assembled capsids.

You can find all AAV particle antibodies here.

In contrast, AAV capsid protein antibodies recognize linear epitopes present on denatured VP proteins.

You can find all AAV capsid protein antibodies here.

Yes, PROGEN offers a selection of AAV antibodies in 10 µg sample sizes. You can find all available AAV antibodies here: AAV Antibodies

PROGEN offers a wide range of AAV antibodies either detecting a linear epitope (AAV capsid protein antibodies), a conformational epitope (AAV particle antibodies) or a AAV replicase.

These antibodies are provided in different quantities and formats (e.g. purified and supernatant). A selection of AAV antibodies are also available in 10 µg sample sizes. You can find all available AAV antibodies here: AAV Antibodies.

Anti-Adeno-Associated Virus Capsid Proteins Antibodies

For more information on the antibody binding epitopes of the capsid protein antibodies A1, B1 and A69 see the table below summarizing the published data from the following publication on the specific antibody binding sites:

Table Binding Sites of the AAV capsid protein antibodies

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Anti-Adeno-Associated Virus Particle Antibodies

For more information on the antibody binding epitopes on assembled AAV1, 2, 5, 8 and 9 capsids, see the tables below summarizing the published data from the following publications on the specific antibody binding sites:

summary binding epitopes


EM structures of assembled AAV1, 2, 4, 5, 6, and 8 capsids with Fab-fragments are available in the following publications:




AlphaLISA® AAV2 Capsid Detection Kit

PerkinElmer has integrated PROGEN’s well-established AAV2 particle antibody A20R into their no-wash AlphaLISA® assay technology. The AlphaLISA® AAV2 Capsid Detection Kit is designed to quantify AAV2 capsids in buffer, cell culture media, cell lysates and cell supernatants.

PROGEN’s A20R antibody exclusively detects fully assembled, intact AAV2 capsids, providing an efficient and reliable quantification of the overall AAV2 capsid titer.

 

View A20R capsid antibody